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polyvinylidine fluoride membrane (Bio-Rad)

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Bio-Rad polyvinylidine fluoride membrane
Polyvinylidine Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyvinylidine+fluoride+membranes/Trans-Blot+SD/pmc11719836-187-24-32
Average 99 stars, based on 3986 article reviews

polyvinylidine fluoride membrane - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Clinical, genetic, and functional characterization of the glycine receptor β-subunit A455P variant in a family affected by hyperekplexia syndrome
Article Snippet: Equal amounts of protein were loaded on premade gels, Bolt 4 to 12%, Bis-Tris (Thermo Fisher Scientific) followed by transfer of proteins onto polyvinylidine fluoride membranes (Biorad).

Article Title: 11β-Hydroxysteroid Dehydrogenase 1 Specific Inhibitor Increased Dermal Collagen Content and Promotes Fibroblast Proliferation
Article Snippet: Thirty micrograms of protein were separated on SDS-polyacrylamide gels and transferred onto polyvinylidine fluoride membranes (Bio-Rad, Hercules, CA, USA).

Article Title: A vitamin D analog inhibits Th2 cytokine- and TGFβ -induced periostin production in fibroblasts: a potential role for vitamin D in skin sclerosis
Article Snippet: Five micrograms of protein were separated on SDS-polyacrylamide gels and transferred onto polyvinylidine fluoride membranes (Bio-Rad, Hercules, CA USA).

Article Title: 11β-Hydroxysteroid Dehydrogenase-1 Is a Novel Regulator of Skin Homeostasis and a Candidate Target for Promoting Tissue Repair
Article Snippet: Ten micrograms of protein were separated on SDS-polyacrylamide gels and transferred onto polyvinylidine fluoride membranes (Bio-Rad, Hercules, CA, USA).

Blocking Assay:

Article Title: Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression
Article Snippet: .. The proteins were transferred onto polyvinylidine fluoride membranes (transblot apparatus, Bio-Rad) and blocked for 30 minutes in blocking buffer (5% non-fat dry milk in 1X TBS 0.1% Tween 20). .. Membranes were then probed with rabbit anti-GLT-1 (C-terminal-directed, 1:5000, final concentration of 0.25μg/mL, Cat#GLT-1a RRID:AB_2314565) ( Rothstein et al., 1994 ), mouse anti-GLAST (1:500, final concentration of 200ng/mL, anti-ACSA-1 pure Miltenyi Biotec 130-095-822 RRID:AB_10829302), rabbit anti-Dll1 (1:500, final concentration of 1μg/mL, abcam ab84620 RRID:AB_1860333), rabbit anti-Dll4 (1:500, final concentration of 2μg/mL, abcam ab7280 RRID:AB_449562), mouse anti-actin (1:10,000, Cell Signaling 3700S RRID:AB_2242334), or a combination of these antibodies in 1% blocking buffer.

Incubation:

Article Title: mTORC1 Signaling in Individual Human Muscle Fibers Following Resistance Exercise in Combination With Intake of Essential Amino Acids
Article Snippet: .. The gels were subsequently incubated in transfer buffer (25 mM Tris base and 192 mM glycine in 90% dH 2 O−10% methanol) for 30 min at 4°C and the proteins transferred to polyvinylidine fluoride membranes (Biorad) at 300 mA for 180 min at 4°C. .. The membranes were subsequently stained using Pierce® Reversible Stain Kit (Thermo Scientific).

Nucleic Acid Electrophoresis:

Article Title: Comparative Transcriptional Analyses in the Nucleus Accumbens Identifies RGS2 as a Key Mediator of Depression-Related Behavior.
Article Snippet: ISS ABSTRACT BACKGROUND: Major depressive disorder is one of the most commonly diagnosed mental illnesses worldwide, with a higher prevalence in women than in men.. Although currently available pharmacological therapeutics help many individuals, they are not effective for most.. Animal models have been important for the discovery of molecular alterations in stress and depression, but difficulties in adapting animal models of depression for females has impeded progress in developing novel therapeutic treatments that may be more efficacious for women.